Human Bronchial Epithelial Cell Line

16hbe14o is a human bronchial epithelial cell line isolated from a 1 year old male heart lung patient and immortalized with the origin of replication defective sv40 plasmid psvori.

Human bronchial epithelial cell line.

16hbe14o human bronchial epithelial cell line 16hbe14o human bronchial epithelial cell line is widely used to model barrier function of the airway epithelium and to study respiratory ion transport as well as the function of cftr. Investigation of this viral infection in the human bronchial epithelial cell line 16hbe suggests that sars cov 2 can enter these cells through interaction between its membrane localized s protein with the angiotensin converting enzyme 2 molecule on the host cell membrane. Primary human bronchial epithelial cells hbepc are isolated from the surface epithelium of human bronchi and stain positive for cytokeratin. Aspirate the supernatant from the tube without disturbing the cell pellet.

Count the cells with a hemocytometer or cell counter. Find msds or sds a coa data sheets and more information. Calu 3 cells human bronchial epithelial cell line are originally derived from the subbronchial epithelium of a lung adenocarcinoma atcc manassas va and have been used as surrogate nasal epithelium due to their similar biophysical properties such as forming tight monolayers and also secreting mucus when cultured in an air liquid. The 16hbe14o cell line was originally developed from human bronchial epithelium to study the cystic fibrosis transmembrane conductance cftr regulator cozens et al 1994 and retains many features of differentiated bronchial epithelial cells.

Resuspend the cells in 2 ml of bronchial tracheal epithelial growth medium by gently pipetting the cells to break up the clumps. Flick the tip of the conical tube with your finger to loosen the cell pellet. The cells are cryopreserved at the second passage to ensure the highest viability and plating efficiency. Atcc primary cell solutions offers epithelial cells isolated from human breast bronchi trachea and small airways cornea prostate and kidneys.

Atcc normal human primary bronchial tracheal epithelial cells when grown in airway epithelial cell basal media supplemented with bronchial tracheal epithelial cell growth kit components provide an ideal cell system to propagate bronchial tracheal epithelial cells in serum free conditions. The cell line retains characteristic features of normal differentiated bronchial epithelial cells including a cobblestone morphology cytokeratin expression the. 16hbe14o cells have a cobblestone appearance in culture form tight junctions and display.