Chromatin immunoprecipitations were performed with cross linked chromatin from nccit cells and either nanog antibody or normal rabbit igg 2729 using simplechip enzymatic chromatin ip kit magnetic beads 9003.
Human nanog antibody cell signaling.
Clone rea314 recognizes the human nanog antigen a homeobox transcription factor also known as es cell associated protein 4.
Anti nanog antibody epr2027 2 ab109250 at 1 2000 dilution lane 1.
Secondary all lanes.
Nanog is thought to be a key factor in maintaining pluripotency.
Goat anti rabbit hrp at 1 2000 dilution developed using the ecl technique.
Nanog in adlf1 and fab2 stem cell lines.
Nanog antibody gt3312 detects nanog protein by western blot analysis.
Mouse embryonic stem cell line d3 lanes 4 5.
Nccit human pluripotent embryonic carcinoma epithelial cell whole cell lysates with 5 nfdm tbst lane 2.
Nanog mrna is present in pluripotent mouse and human cell lines and absent from differentiated cells.
Hela human cervix adenocarcinoma epithelial cell whole cell lysates with 5 nfdm tbst lane 3.
Hek 293 human embryonic kidney epithelial cell whole cell lysates with 5 nfdm tbst.
Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues near the amino terminus of human nanog protein.
Nanog was detected in immersion fixed adlf1 top panel and fab2 bottom panel induced pluripotent stem cell lines using goat anti human nanog antigen affinity purified polyclonal antibody catalog af1997 at 10 µg ml for 3 hours at room temperature.
1 1000 the hrp conjugated anti mouse igg antibody gtx213111 01 was used to detect the primary antibody.
Nanog expression is controlled by a network of factors including sox2 and the key pluripotency regulator oct 4 1.
Recent advances in somatic cell reprogramming have utilized viral expression of combinations of transcription factors including nanog oct 4 sox2 klf4 c myc and lin28 2 3.
This antibody reacts with human samples.
Background nanog is a homeodomain containing transcription factor that is essential for the maintenance of pluripotency and self renewal in embryonic stem cells 1.
Mutant mouse embryonic stem cell line known to not express nanog lysates proteins at 200000 cells per lane.
Human nanog protein shares 52 overall amino acid identity with the mouse protein and 85 identity in the homeodomain.
Tested in western blot wb immunofluorescence if immunocytochemistry icc and immunohistochemistry paraffin ihc p applications.
Anti nanog antibody ab80892 at 1 500 dilution lane 1.
Invitrogen anti nanog monoclonal hnanog 2 ebioscience catalog 14 5768 82.
30 μg human esc whole cell lysate extract 10 sds page nanog antibody gt3312 gtx627421 dilution.
It is expressed in the central portion of the morula the inner cell mass of the blastocyst in embryonic stem cells esc and embryonic germ cells egc.
Marker lanes 2 3.
Supplied as 100 µg purified antibody 0 5 mg ml.
2008 cell 132 1049 61.
